Detectx 2 3 cyclic gamp Elisa kit

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The Abcam Complete Cyclic GMP In Vitro Competitive ELISA Enzyme-Linked Immunosorbent Assay Kit is designed for the precise quantitative measurement of cyclic complete GMP in cells and tissues treated with 0.1N HCl, as well as culture supernatants, saliva, and serum.

A goat anti-rabbit IgG antibody has been precoated in 96-well plates. Standards or test samples are added to the wells, along with alkaline phosphatase (AP) -conjugated cGMP antigen and a cGMP-specific rabbit polyclonal antibody. After incubation, excess reagents are washed off.

Papp substrate is added and after a short incubation the enzymatic reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow staining is inversely proportional to the amount of cGMP captured on the plate.

Cyclic 3 ‘, 5’-guanosine monophosphate (cyclic GMP; cGMP) was identified in 1963. It has been shown to be present at levels typically 10-100 times lower than cAMP in most tissues and is formed by enzyme action.

guanylate cyclase in GTP. It is involved in a number of important biological reactions. Some hormones, such as acetylcholine, insulin, and oxytocin, as well as some other chemicals such as serotonin and histamine, cause cGMP levels to rise.

Guanylate cyclase stimulators such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels. Peptides, such as atrial natriuretic peptide (ANP), which relax smooth muscle, also increase cGMP concentrations. cGMP has been confirmed as ANP’s second messenger.

NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells.

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